Step-by-Step Instructions for Mixing Research Peptides
How to reconstitute lyohilized (freeze dried) peptides with the bacteriostatic water
Peptide vial, bacteriostatic water, sterile syringes, and alcohol wipes.
Remove the protective cap from both the peptide vial and the bacteriostatic water vial without disturbing the rubber stoppers.
Clean the rubber stopper of both vials thoroughly using alcohol wipes and allow them to air dry.
Turn the bacteriostatic water vial upside down and draw the required amount of water into the syringe according to your research protocol.
Hold the peptide vial at a slight angle, gently insert the needle, and slowly inject the water along the inside wall of the vial.
Do NOT shake. Gently roll or swirl the vial until the lyophilized powder fully dissolves and the solution becomes clear.
Store the reconstituted peptide in a refrigerator and use within 45 days of reconstitution.
When preparing research compounds, the amount of liquid used for reconstitution is flexible. What truly matters is the dose you measure when drawing from the vial. Adding more or less bacteriostatic water simply changes the concentration, not the total amount of compound in the vial. For example, a 10 mg vial will always contain 10 mg total, whether you add 1 mL, 2 mL, or 3 mL of solution. The only difference is how many syringe units are required to reach a specific dose.
Final Concentration (mg per mL)
| Vial Amount | 1 mL | 2 mL | 3 mL | 4 mL | 5 mL |
|---|---|---|---|---|---|
| 5 mg | 5 mg/mL | 2.5 mg/mL | 1.67 mg/mL | 1.25 mg/mL | 1 mg/mL |
| 10 mg | 10 mg/mL | 5 mg/mL | 3.33 mg/mL | 2.5 mg/mL | 2 mg/mL |
| 15 mg | 15 mg/mL | 7.5 mg/mL | 5 mg/mL | 3.75 mg/mL | 3 mg/mL |
| 20 mg | 20 mg/mL | 10 mg/mL | 6.67 mg/mL | 5 mg/mL | 4 mg/mL |
| 25 mg | 25 mg/mL | 12.5 mg/mL | 8.33 mg/mL | 6.25 mg/mL | 5 mg/mL |
| 30 mg | 30 mg/mL | 15 mg/mL | 10 mg/mL | 7.5 mg/mL | 6 mg/mL |
Each box shows the micrograms delivered per 10 units on the syringe. (1000mcg equals 1mg. EX. 2000mg = 2mg, 2500mcg= 2.5mg)
| Vial Size | 1 mL | 2 mL | 3 mL | 4 mL | 5 mL |
|---|---|---|---|---|---|
| 5 mg | 500 mcg / 10u | 250 mcg / 10u | 167 mcg / 10u | 125 mcg / 10u | 100 mcg / 10u |
| 10 mg | 1000 mcg / 10u | 500 mcg / 10u | 333 mcg / 10u | 250 mcg / 10u | 200 mcg / 10u |
| 15 mg | 1500 mcg / 10u | 750 mcg / 10u | 500 mcg / 10u | 375 mcg / 10u | 300 mcg / 10u |
| 20 mg | 2000 mcg / 10u | 1000 mcg / 10u | 667 mcg / 10u | 500 mcg / 10u | 400 mcg / 10u |
| 25 mg | 2500 mcg / 10u | 1250 mcg / 10u | 833 mcg / 10u | 625 mcg / 10u | 500 mcg / 10u |
| 30 mg | 3000 mcg / 10u | 1500 mcg / 10u | 1000 mcg / 10u | 750 mcg / 10u | 600 mcg / 10u |
